engleski

Interaction of GSM modulated RF radiation and macromolecular cytoskeleton structures

The aim of study was to investigate whether there is an interaction between modulated radiofrequency radiation (RFR) and macromolecular cytoskeleton structures ; microtubule, actin and vimentin filaments. Continuous cell line of Chinese hamster fibroblasts were exposed to global system of mobile (GSM) modulated electromagnetic field at frequency of 915 MHz, electric field strength of 30 V/m. Average specific absorption rate (SAR) was calculated at 1, 6 W/kg. Cells were exposed for 1, 2, and 3 hours in specially constructed Gigahertz Transversal Electromagnetic Mode cell (GTEM-cell). Negative- and positive cell controls were included into the experiment. Trypan blue exclusion test was used to determine cell viability. The structure of microtubule and vimentin proteins was determined by indirect immunocytochemistry method whereas structure of actin microfilaments was rhodamine-phalloidin marked and microscopically analyzed. Viability in exposed cells did not differ from the matched controls. Microtubule structure in treated cells was significantly altered after two and three hours, while actin microfilaments were altered only after three hours of exposure. Vimentin filaments remained unchanged throughout the course of experiment. It could be concluded that dissimilar to non-polar vimentin, applied RF radiation destructively interact with polar cytoskeleton structures - microtubules and actin proteins.

915 MHz; GTEM cell; V79 cell culture; immunochemistry; microtuble; actin; vimentin

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