engleski

Autochtonous functional starter cultures and mycotoxins in "Slavonski kulen"

The purpose of this study was to isolate the indigenous microbial population from "Slavonski kulen", produced in rural households. Dominant microbial populations consisted of lactic acid bacteria (LAB): L.plantarum, L.delbrueckii, Leuconostoc mesenteroides and L. acidophilus in number from 3.0-9.23 log10 CFU/g sample. The bacteria from the genous of Staphylococcus was also dominanted microbial population in "Slavonski kulen", as follows: S. xylosus, S. warneri, S. lentus and S. auricularis in number from 3.30-5.56 log10 CFU/g sample. From samples were also isolated yeasts: Candida famata. From the surface 4 of 6 analyzed "Slavonski kulen" were isolated molds from the genus Penicillium sp. and Aspergillus sp. The presence of mycotoxins were also determined. Mycotoxins concentrations were 0.9 to 1.6 ppb for ochratoxin A (OTA) and 0.1 to 0.5 ppb for aflatoxin B1 (AFB1) and were defined not only on the surface layer, but also in the centre of "Slvonski kulen". In the samples which were not isolated molds, it was not proven the presence of mycotoxins. All LAB isolates showed significant antimicrobial activity against tested pathogenic microorganisms, and showed one of the important properties of potential starter cultures.

mycotoxins; starter cultures; Slavonski kulen

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