engleski

Gene promoter methylation profiles in clarifying carcinogenicity of HPV 53 and HPV 66

Our previous studies indicated that the prevalence of common human papillomavirus (HPV) types in Croatian women decrease with age, while the prevalence of rare HPV types increase. In a particular study group, women age 55 and over, there were more undetermined HPV types than in the other age groups. Upon genotyping of these undetermined HPV types, rare HPV types such as HPV 53 and HPV 66 were surprisingly abundant, found in 10.3% and 3.7% cases, respectively. The prevalence of the common HPV types in this study group was lower compared to HPV 53 (HPV 31 found in 7.4% cases ; HPV types 16 and 33 found in 4.4% cases each) and HPV 66 (HPV types 6/11, 52, 58 and 45 found in less than 3.7% cases each). According to the last epidemiological classification HPV types 53 and 66 still have unclear carcinogenic potential. Therefore in this study, we focused on HPV 53 and HPV 66 positive cervical samples and analyzed the methylation status of the promoter of different host cell genes. Methylation specific PCR (MSP) with primers specific for methylated and unmethylated forms of the gene was used to amplify bisulfite converted DNA. Correlation between gene promoter methylation profiles of cervical samples and cytological diagnosis was assessed. Among all tested gene promotors, DAPK1 and RARβ2 methylation profiles seem to be the most informative in these samples.

human papillomavirus; methylation

nije evidentirano