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Expression, purification and crystallization of wheat profilin (Tri a 12)

Wheat profilin, designated Tri a 12, has recently been found to be recognized by specific IgE antibodies in patients suffering from bakers' asthma, wheat induced food allergy and also in patients with grass pollen allergy. Profilins are one of the most prevalent plant protein families causing IgE mediated reactions. Since profilin sequences are highly conserved among plants, individuals who are sensitized to profilin commonly show allergic symptoms to a large number of unrelated plants. The recombinant 14 kDa protein was produced in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. A data set diffracting to a resolution of 3.3 Å was collected in-house from a single crystal. The crystals belonged to space group P3221, with unit-cell parameters a = b = 58.9 Å, c = 82.5 Å, α = β = 90° and γ = 120°.

wheat profilin; expression; crystallization; food allergy

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