Nalazite se na CroRIS probnoj okolini. Ovdje evidentirani podaci neće biti pohranjeni u Informacijskom sustavu znanosti RH. Ako je ovo greška, CroRIS produkcijskoj okolini moguće je pristupi putem poveznice www.croris.hr
izvor podataka: crosbi

The phenanthridine biguanides efficiently differentiate between dGdC, dAdT and rArU sequences by two independent, sensitive spectroscopic methods (CROSBI ID 169756)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Radić Stojković, Marijana ; Miljanić, Snežana ; Mišković, Katarina ; Glavaš-Obrovac, Ljubica ; Piantanida, Ivo The phenanthridine biguanides efficiently differentiate between dGdC, dAdT and rArU sequences by two independent, sensitive spectroscopic methods // Molecular biosystems, 7 (2011), 5; 1753-1765. doi: 10.1039/C1MB05030C

Podaci o odgovornosti

Radić Stojković, Marijana ; Miljanić, Snežana ; Mišković, Katarina ; Glavaš-Obrovac, Ljubica ; Piantanida, Ivo

engleski

The phenanthridine biguanides efficiently differentiate between dGdC, dAdT and rArU sequences by two independent, sensitive spectroscopic methods

At submicromolar concentrations two novel phenanthridine biguanides exhibit distinctly different spectroscopic signals for dGdC and dAdT sequences, respectively, by opposite fluorimetric changes (quenching for dGdC and increase for dAdT) and especially bis-biguanide derivative gives opposite ICD response (negative ICD for dGC and strong positive for dAdT). This specific signalling was explained by ability of compounds to switch binding mode from intercalation into dGdC to minor groove binding into dAdT sequences. Both compounds bind to rArU by intercalation, yielding different fluorimetric and CD response in comparison to any of aforementioned ds-DNA. Moreover, both compounds revealed significantly higher affinity toward ds-polynucleotides in comparison to previously studied alkylamine- and urea-analogues. Furthermore, DNA/RNA binding properties of novel compounds could be controlled by pH, due to the protonation of heterocyclic nitrogen. Low in vitro cytotoxicity of both compounds against human cell lines makes them interesting spectrophotometric probes.

phenanthridine; biguanide; DNA; RNA

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

Podaci o izdanju

7 (5)

2011.

1753-1765

objavljeno

1742-206X

10.1039/C1MB05030C

Povezanost rada

Kemija

Poveznice
Indeksiranost