Soil microbial diversity : an ISO standard for soil DNA extraction (CROSBI ID 171493)
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Podaci o odgovornosti
Philippot, Laurent ; Abbate, Cristina ; Bispo, Antonio ; Chesnot, Thierry ; Hallin, Sara ; Lemanceau, Philippe ; Lindström, Kristina ; Pandard, Pascal ; Romero, Esperanza ; Schloter, Michael ; Simonet, Pascal ; Smalla, Kornelia ; Wilke, Berndt-Michael ; Petrić, Ines ; Martin- Laurent, Fabrice
engleski
Soil microbial diversity : an ISO standard for soil DNA extraction
We proposed in 2006 the standardization of soil DNA extraction to the International Organization for Standardization (ISO), with support from the French Standards Association (AFNOR) and the French Environment and Energy Management Agency (ADEME). The need for an international standard for soil DNA extraction has been rapidly recognized by ISO members as a priority and formally agreed upon. The ISO 11063 Soil quality - Method to directly extract DNA from soil samples is now being prepared by the Technical Committee ISO/TC 190, Subcommittee SC4, Biological methods. The standard was developed based on the publication made by Martin-Laurent et al. (Martin- Laurent et al. 2001). In the first phase, the technical scope of the future standard has been defined by a working group, consisting of expert researchers from countries interested in the subject matter. It was decided that since DNA purification is dependent on soil type, only the actual DNA extraction step, which is likely to generate the main differences between methods, would be subject to standardization. After a successful French inter-laboratory assay, we were requested during the ISO/TC 190 meeting in Delft in November 2008 to set up an international ring- test for a final validation of the method. This task involved ten different laboratories from seven countries and the final report of this ring test was sent to the ISO in January 2010.
DNA extraction ; soil ; microbial community
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Podaci o izdanju
10 (7)
2011.
1344-1345
objavljeno
1439-0108
1614-7480
10.1007/s11368-010-0265-8