engleski

CacO-2 cell uptake ofF Ca, Mg and Fe from biscuits as affected by enrichment with pseudocereal/inulin mixtures

In vitro bioavailability/Caco-2 cell culture system was used to assess calcium, magnesium and iron bioavailability from different types of biscuits enriched with the mixtures of different types of pseudocereals and legumes with inulin. Comparisons were made with the wheat flour based biscuit as a reference sample, in order to evaluate used approach for developing functional biscuit with improved mineral content, bioavailability and uptake. Significant increase of mineral content was achieved in all modified biscuits in relation to reference sample. Average bioavailability of investigated minerals ranged from 41.1% (Fe and Ca) up to 71.2% (Mg). Caco-2 cell uptake of investigated elements, ranged from 7.13% to 12.20% (Fe) ; from 26.39% to 38.95% (Ca) and from 19.65% to 27.17% (Mg). Inulin was determined as an important promoter of Ca and Mg bioavailability. Polyphenols and dietary fiber were determined as the principal components impairing the uptake of Fe and Ca. Enrichment of the reference recipe with the mixture of inulin with soy or amaranth flour most positively influenced bioavailability/uptake of investigated minerals.

Caco-2 cell culture; bioavailability; biscuit; calcium; magnesium; iron

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