engleski

Development, characterization and cross-amplification of microsatellite markers from common sage (Salvia officinalis L., Lamiaceae)

The common sage (Salvia officinalis L.) is a native plant of northern and central Spain, southern France and western part of the Balkan Peninsula. Because of its medicinal and aromatic characteristics it is often gathered from the wild. The assessment of genetic diversity using molecular markers is a starting point for its introduction into plant breeding programs and agricultural production. Eleven new microsatellite markers were developed from genomic DNA libraries enriched for GA and GT repeats. 25 common sage plants from a natural population from the eastern Adriatic coast were examined for polymorphism using the developed microsatellites markers. A total of 142 alleles were observed across eleven loci, the number of alleles per locus ranged from eight to 26. The observed heterozygosity ranged from 0, 300 to 0, 833, and the expected heterozygosity from 0, 666 to 0, 934. 7 out of eleven microsatellites showed high polymorphic information content (PIC) of more than 0, 75 indicating that these microsatellite markers are very useful in assessing genetic diversity and population structure. All eleven microsatellite markers were tested for cross-amplification in four different Salvia species and Rosmarinus officinalis L. The species with most successful amplification was Salvia fruticosa Mill.. (60%), while in other tested species, amplification rate was: 25 % for S. pratensis L., 20 % for S. sclarea L., 20% for S. verticillata L. and 20% for R. officinalis L..

Dalmatian sage; new microsatellites; cross-amplification

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