engleski

A Continuous Spectrophotometric Assay for Measuring Amino Acid Activation by Peptide Synthetases

Background and purpose: Peptide synthetases are multifunctional enzyme complexes catalysing the non-ribosomal biosynthesis of a variety of bioactive peptide products. The activation reaction is generally monitored using the isotope exchange assay, which suffers several disadvantages such as discontinuous monitoring and the use of radioactive reagents. A continuous spectrophotometric assay was introduced for the measurement of PPi generation accompanying the amino acid activation reaction catalysed by peptide synthetases.

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