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Glutathione concentration and glutathione S-transferase activity in gynecological normal and tumor tissues: a preliminary report

Drug resistance is a major problem in cancer tfherapy. In vitro studies have suggested that glutathion (GSH) and glutathione transferase (GST) may be associated with alkylating agents resistance. In this study, we determined GSH concentrations and GST activity in 41 samples of gynecologic tissues which include: normal tissue of cervix uteri (7 samples), normal tissue of corpus uteri (14 samples), benign tumors of corpus uteri (4 samples), malignant tomors of corpus uteri (7 samples), the normal tissue of ovary (5 samples), benign ovarian tumors (2 samples) and 2 malignant ovarian tumors. The GSH concentrations were similar in normal tissue of corpus uteri, but these levels were lower in benign tumors. The GST activities were similar in the normal tissue of cervix uteri, corpus uteri and ovary. In corpus uteri, similar values were obtained for normal tissue and benign tumors, but higher ones for malignant tumors. This difference was statistically significant if two malignant Muller mixed tumors (with very low GST levels) were excluded from the analysis. Similar GST activities were obrained for the normal ovarian tissue and benign ovarian tumor, but higher activities for ovarian malignant tumors. In spite of a modest number of samples, our data nevertheless suggest that the activity of glutathione transferase is incresased in tumor tissues. The GST activity may contribute to resistance of tumor cells to alkylating drugs in chemotherapy.

ovarian neoplasms-analysis ; uterine neoplasms-analysis ; glutathione ; glutathione transferases ; drug resistance

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