engleski

ASSESSMENT OF BARLEY BREEDING GERMPLASM BY SSR MARKERS ASSOCIATED WITH CERTAIN QTL’S REGARDING ABIOTIC STRESS TOLERANCE AND QUALITY

Breeders around the globe consider breeding for abiotic stress tolerance as something that preoccupies their attention more than ever before. Also, the malting industry has some specific demands towards barley quality improvement. A total of 126 barley cultivars, 78 winter and 48 spring type from different background were screened by SSR markers in order to assess genetic variability among them. Croatian barley cultivars are represented with 66 winter and 27 spring type (total 93). Preliminary data presented in this paper is derived for markers Bmac181 (chr. 4H), Bmag211 (chr. 1H), EBmac755 (chr. 7H) and HVM54 (chr. 2H). All of them are linked to certain QTLs, either for abiotic stress tolerance or quality, published in a number of studies done on different populations. Specified QTLs are for drought tolerance, (pinpointed by markers Bmac181 and EBmac755), viscosity (Bmag211) and hull content (HVM54). The number of alleles per SSR locus averaged 5.5 among elite breeding lines. A number of cultivars for which specific allele amplification has been successful, were selected and their distribution displayed. This type of assay will be useful for determination of the informativeness level of SSR markers used when generating a meaningful classification of elite germplasm. Also, some pointers considering carriers of QTLs of interest can be highlighted for future selection of parental lines in hybridization process.

barley; abiotic stress; quality; SSR; QTL

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