engleski

Genetic variability of Croatian Citrus tristeza virus isolates

About 30 citrus field samples were collected from different orchards in the Croatian coastal region. The majority of trees was grafted on Poncirus trifoliata and displayed no symptoms of Citrus tristeza virus (CTV) infection. However, about 50% of tested trees were found CTV-infected. The genetic variability and population structure of CTV were studied by SSCP and sequence analysis of the cloned p25 (CP) and p23 genes. The results of phylogenetic analysis, corroborated by biological characterization, showed that both CP and p23 sequences clustered into phylogenetic groups that include sequences of severe quick decline, seedling yellows and stem pitting isolates. Evidence of mixed infections and recombination events was also found.

CP gene; CTV; phylogenetic analysis; SSCP

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