engleski

EFFECTS OF PPARγ, APOE, ACE AND AT1R GENE VARIANTS ON DEVELOPMENT OF METABOLIC SYNDROME

Background. Metabolic syndrome (MS) is a cluster of risk factors including hypertension, abdominal obesity, dyslipidemia and hyperglycemia. The contribution of genetic factors to the development of MS has been widely recognized, but the contribution of the genes has not yet been fully clarified. We investigated the possible role of gene polymorphisms of PPAR (Pro12Ala), ApoE, ACE (I/D) and AT1R (1166A>C) in MS. Methods. Genotyping of PPARG2, AT1R and ACE was performed by PCR-RFLP, APOE by real-time PCR in group of 281 patients and 119 controls. Associations of alleles and genotypes with biological and clinical variables were performed using UNPHASED-3.0.10. Results. In comparison to females, males were older, had higher BMI and waist circumference, higher triglycerides and glucose levels and lower HDL. Males had significantly more often high blood pressure. Age accounted for the differences in glucose levels and HDL. We found significant association of AT1R variants and the developement of MS (p=0.03), with the C allele being associated with lower risk. We found association of waist circumference with: ACE variants (p=0.03), with D allele carrying the higher risk ; AT1R with A as the risk allele (p=0.05). APOE4 variant was associated with the waist circumference (p=0.05) and the levels of HDL (p=0.03), and ACE genotype was associated with glucose levels (p=0.02), with II genotype carrying the lowest risk. Conclusion. Gene variants of AT1R, ACE and ApoE could be susceptibility factors of obesity, glucose intolerance and lipid status contributing to the development of metabolic syndrome.

metabolic syndrome; PPARG; ApoE; ACE; AT1R

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