engleski

Temperature-dose relationships with aflatoxin M1 in milk on the brine shrimp (Artemia salina) larvae

Temperature-dose relationships with aflatoxin M1 (AFM1) were studied using the brine shrimp Artemia salina larvae as an biological indicator in the temperature range from 20 oC to 40 oC. Increase in the incubation temperature resulted in sensitivity increase by the brine shrimp to AFM1. Optimum sensitivity occured at 30 oC. Positive results were obtained at 0.18 µg AFM1 x L-1 of whole pasteurized milk with a mortality of over 15%. Greater than 90% mortality occurred at dose levels of 0.9 µg AFM1 x L-1 and above. The test can be conducted during 30-60 hours.

aflatoxin M1; bioassay; Artemia salina; LC50 and T50 doses

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