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Genetic Mapping and Quantitative Trati Loci (QTL) Analysis in Faba Bean (Vicia Faba L.)

The genetic linkage maps constitute an essential tool in many areas of plant genetics and breeding. Using genetic maps, it is possible to estimate the number of loci controlling genetic variation of the trait of interest in a segregating population and to analyse the effects of these loci. Faba bean (Vicia faba L.) possesses one of the largest genomes among legumes (~13, 000 Mb), compared with other better-characterized legume crops as soybean, pea or Medicago truncatula Gaertn. The large genome size seriously complicates the development of saturated linkage maps, as well as the identification of important genes. Nevertheless, combining information gathered by segregation analyses of a range of marker systems (morphological, isozyme, RAPDs, SSRs, STSs, ESTs, SCARs) in populations from different crosses and generations (F2, RILs) and by including families derived from primary trisomics it has been possible to develop the saturated, consensus map of the faba bean genome. The genetic map has been successfully used in the analysis of quantitative trait loci (QTLs) conferring resistance to broomrape (Orobanche crenata Forsk.) and to ascochyta blight (caused by Aschochyta fabae Speg.). The accurate identification of genomic locations of individual QTLs controlling resistance is of utmost importance in order to develop marker-assisted selection (MAS) programmes.

faba bean; molecular markers; genetic map; QTLs; marker-assisted selection

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