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Evaluation of steroid hormones anabolic use in cattle in Croatia (CROSBI ID 172446)

Prilog u časopisu | izvorni znanstveni rad

Pleadin, Jelka ; Terzić, Svjetlana ; Nina, Perši ; Vulić, Ana Evaluation of steroid hormones anabolic use in cattle in Croatia // Biotechnology in animal husbandry, 27 (2011), 2; 147-159

Podaci o odgovornosti

Pleadin, Jelka ; Terzić, Svjetlana ; Nina, Perši ; Vulić, Ana

engleski

Evaluation of steroid hormones anabolic use in cattle in Croatia

The hormone concentration exceeding the physiological ones, found on monitoring fot illegal substance use, would point to the abuse of these substances for anabolic purpose. In the present study, concentration of the natural hormones 17 beta-estradiol, progesterone and testosterone were determined in bovine plasma according to animal sex and age. Natural hormone concentration were determined using quantitative validated ELISA methods in plasma samples from cattleof different breed composition collected at several farms in Croatia during the 2006-2009 period. Method validation showed good mean recovery and repeatibility (approx. 75-87%), demonstrating the methods efficacy in determination of 17 beta-estradiol, progesterone and testosterone level in cattle plasma, respectively. The level of sex hormones was statistically significantly higher in yearling plasma as compared with plasma as compared with calf plasma (P<0.05). The highest levels of 17 beta-estradiol (0.03+/-0.01 ng/ml)and progesterone (4.87+/-5.47 ng/ml) in male yearlings. Results showed the steroid hormone levels to vary with animal age and sex, indicated that illegal use of anabolic substances could not be suspected in none of study animals.

steroid hormones ; anabolics ; physiological levels ; plasma ; bovine

nije evidentirano

nije evidentirano

nije evidentirano

nije evidentirano

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Podaci o izdanju

27 (2)

2011.

147-159

objavljeno

1450-9156

2217-7140

Povezanost rada

Veterinarska medicina, Biotehnologija