engleski

Evaluation of antioxidant treatment as a new prevention model for kidney stone formation

Urolithiasis is a disease characterized by formation of solid deposits in the urinary tract. The numerous causes may lead to kidney stone formation. However, most kidney stones are predominantly composed of calcium oxalate which predominantly generates free radicals that are damaging to the renal tubular cells To study the toxic effect of oxalate to renal tubular cells and possible inhibition of oxalate caused oxidative stress by antioxidant treatment. Madine-Darby canine kidney cells (MDCK cells) were used as in vitro model of urolithiasis. Oxidative stress was induced by exposure of MDCK cells to sodium oxalate (NaOX). In order to prevent oxidative stress MDCK cells were pretreated with different concentrations of L-arginin, an antioxidant. The cytotoxicity of oxalate against MDCK cells was determined by MTT Assay and by light microscopy. The oxidative stress was evaluated by expression of superoxide dismutase (SOD) by RT PCR using RNAeasy kit (Qiagen) and standard protocol. In the cells treated with NaOX only, observed necrosis was proportional to the concentration of NaOX. Interestingly, cells treated with L-arginin prior NaOX exposure showed lover levels of necrosis by light microscopy as well as by MTT assay. Positive correlation of SOD expression was observed in all groups of cells by RT-PCR. Our results suggest that L-arginin is able to hamper oxalate induced oxidative stress generation in kidney cells and as such may play role in prevention of urolithiasis. More studies are needed to further evaluate its potential as a prevention agent of urolithiasis.

urolithiasis ; L-arginin ; MDCK-II ; prevention ; oxidative stress

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