engleski

Haemolymph biochemistry profile of lobster Palinurus elephas (Fabricius, 1787) after exposure to emersion

Live lobsters Palinurus elephas (Fabricius, 1787) were collected from a number of sites around the island of Vis, and transported to laboratory exposed to air for considerable time. The aim of the study was to examine the effect of emersion on the plasma biochemical profile which is a useful indicator of physiological status. Lobsters had average body weight of 517, 09 g and carapace length of 113, 20 mm. Haemolymph was withdrawn from ventral abdominal artery. Biochemical procedures were carried out to study metabolites (trygliceride, TRIG ; cholesterol, CHOL ; total proteins, TP ; glucose, GLU ; urea, BUN ; creatinin, CREA) and several enzymes (alkaline phosphatase, ALKP ; aspartate aminotransferase, AST ; gamma glutamyltransferase, GGT ; lactate dehydrogenase, LDH). In addition to classic statistical analyses, six machine-learning methods utilizing ten times ten-fold cross-validation evaluation were used in order to determine the best classification model for the blood biochemistry data. Blood GLU, BUN, ALKP, TP significantly increased while CREA was significantly lower. This approach clarifies the role and importance of physiological processes which show diversity of functional characteristics of various biochemical parameters in case of long term exposure to air.

haemolymph biochemistry; Palinurus elephas

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