Final step in energy conductance from thylakoid complexes to stromal reducing equivalents as a source of metabolic retrograde signals (CROSBI ID 575678)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Fulgosi, Hrvoje ; Vojta, Lea ; Jurić, Snježana ; Tomašić Paić, Ana
engleski
Final step in energy conductance from thylakoid complexes to stromal reducing equivalents as a source of metabolic retrograde signals
Working in synchrony, photosynthetic charge separation, electron transfer, and redox reactions generate proton motif force necessary for the synthesis of ATP and transport of electrons toward stromal reducing equivalent NADPH. The last step of electron transfer from ferredoxin to NADP+ is catalyzed by ubiquitous flavin adenine dinucleotide (FAD)-binding enzyme, ferredoxin-NADP+ oxidoreductase (FNR). FNR has been implicated in various thylakoid energy transduction pathways ranging from cyclic electron flow to regulation and management of oxidative stress. Two proteins, TROL (thylakoid rhodanese-like) and Tic62 (62 kDa component of the translocon at the inner envelope of chloroplasts) have been characterized and shown to form dynamic complexes with FNR. Being multi-pass membrane protein, TROL qualifies for the long-sought membrane anchor of FNR. Both TROL and Tic62 can also be found at chloroplast inner envelope, revisiting the notion of electron transfer chain specific for this compartment. Tethering of FNR to Tic62/TROL is accomplished via a conserved Ser/Pro-rich motif which forms type II alpha-helix and presumably mediates the interaction in pH-dependent manner. Inactivation of TROL leads to changes in efficiency of electron transfer and induction of non-photochemical quenching. TROL-deficient plants have changed nuclear gene expression with up-regulation of NADPH-dependent malic enzyme, which can form NADPH in an alternative pathway. Thus, NADPH synthesis, mediated by FNR-TROL interaction, may be the source element in metabolic retrograde signal-transduction pathway linking light reactions with nuclear gene expression.
photosynthesis; FNR; NADPH; redox regulation
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Podaci o prilogu
53-53.
2011.
objavljeno
Podaci o matičnoj publikaciji
Abstract Book
Vothknecht, Ute ; Teige, Markus ; Rocha, Agostinho G. ; Depauw, Frauke
München: Ludwig-Maximillians Universitaet
Podaci o skupu
"Plant Organellar Signaling from Algae to Higher Plants" FEBS workshop and final symposium of the Marie-Curie ITN COSI
pozvano predavanje
31.08.2011-03.09.2011
Primošten, Hrvatska