Comparison of ractopamine and clenbuterol accumulation in pigs as meat production animals (CROSBI ID 575736)
Prilog sa skupa u zborniku | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Pleadin, Jelka ; Vulić, Ana ; Perši, Nina ; Vahčić, Nada ; Milić, Dinka
engleski
Comparison of ractopamine and clenbuterol accumulation in pigs as meat production animals
Ractopamine and clenbuterol are β-adrenergic agonists that increase the rate of weight gain and feed conversion. If used in meat production, they promote muscle growth of treated animals by increasing nitrogen retention, protein synthesis and lipolysis, and suppressing lipogenesis. They can be illegally used during fattening of farm animals, thus implying a risk to consumers of meat and meat products. For residue surveillance of both substances, it is essential to choose appropriate matrices and use high sensitivity analytical methods in the analysis of suspect tissues. The aim of the study was to compare residue levels and accumulation potential of ractopamine and clenbuterol in pig tissues, liver and kidney (n=24), after subchronic repeat administration of clenbuterol and 10-fold dose of ractopamine. Ractopamine and clenbuterol residues in both matrices were determined using validated enzyme-linked immunosorbent assay (ELISA) as a quantitative screening method. Method validation showed good mean recoveries (R >70%) with acceptable inter- and intra-day relative standard deviations (RSD <10%) for both analytes and matrices. Immediately after the last dose of β-agonists, the maximal determined ractopamine concentration in the kidney (42.44 ng/g) was 2-fold that measured in the liver (20.66 ng/g) and was comparable with maximal concentration of clenbuterol in the liver (47.43 ng/g). Considering the dose difference, study results pointed to higher accumulation of clenbuterol residues in both matrices, suggesting the liver to have a significantly higher accumulation potential for clenbuterol residues and kidney for ractopamine residues. The results also suggested these tissues to be appropriate matrices to detect ractopamine and clenbuterol abuse in meat production.
ractopamine; clenbuterol; accumulation; tissues of pigs; meat production
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Podaci o prilogu
317-321.
2011.
objavljeno
Podaci o matičnoj publikaciji
Proceedings of the 7th International Congress of Food Technologists, biotechnologists and nutritionists
Medić, Helga
Zagreb: Hrvatsko društvo prehrambenih tehnologa, biotehnologa i nutricionista
978-953-99725-4-5
Podaci o skupu
7th International Congress of Food Technologists, biotechnologists and nutritionists
poster
20.09.2011-23.09.2011
Opatija, Hrvatska