engleski

Adhesion of Zearalenone by lactic acid bacteria

INTRODUCTION: Mycotoxins are natural toxins with very different chemical structures that are produced by moulds. People and animals are exposed to the mycotoxins mainly through food, mostly through the moulds contaminated grain cereals. Zearalenone is a natural metabolite of several species of fungi from the genus Fusarium. The main contaminant of corn, but can be found in wheat, barley, oats, rice, baked in bread, beans and hay. Today, there are requires of less use of chemical compounds in the preservation and protection of food. Lactic acid bacteria are a natural way of protecting food and feed, and thereby don't have bad effects on the environment. METHODOLOGY: In this paper is accompanied binding of zearalenone by lactic acid bacteria for 72 hours at a temperature of 27 oC in MRS-broth. Measuring of the concentration of zearalenone by ELISA method in the remaining medium in which lactic acid bacteria Lactobacillus plantarum L4 and Lactobacillus rhamnosus GG were added the ability of these bacteria for binding of this mycotoxin was determined. RESULTS AND DISCUSSION: Binding of zearalenone by bacterium Lactobacillus plantarum L4 (concentration of 108 CFU x mL-1, 0 hours) was almost 100%, while at concentrations of 104 CFU x mL-1 and 106 CFU x mL-1 the ability was slightly declining. Binding capacity of zearalenone by bacteria Lactobacillus rhamnosus GG at concentrations of 104 CFU x mL-1, 106 CFU x mL-1 and 108 CFU x mL-1 in 0 hours was reduced by approximately 15% in relation to the ability exhibited by the bacteria Lactobacillus plantarum L4 in the same conditions. During the further time of incubation binding capacity decreases. CONCLUSIONS: From these results it was found that the most efficient binding of mycotoxins with lactic acid bacteria takes place immediately after their addition, and is associated with concentration of cells of LAB.

ELISA; Lactobacillus plantarum L4; Lactobacillus rhamnosus GG; MRS-broth; Zearalenone

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