Effect of bisperoxovanadate-1,10-phenantroline on growth and differentiation in tumor cell lines (CROSBI ID 464418)
Prilog sa skupa u zborniku | izvorni znanstveni rad
Podaci o odgovornosti
Cerovac, Željka ; Kovač, Višnja ; Ban, Jasna
engleski
Effect of bisperoxovanadate-1,10-phenantroline on growth and differentiation in tumor cell lines
Bisperoxovanadate-1,10-phenantroline, bpV, is an insulin mimetic in cultured cells. This compound activated the insulin receptor kinase and inhibited dephosphorilation of autophosphorilated insulin receptors. This compound is the most potent phosphotyrosine phosphatase inhibitor described to date. bpV induced differentiation determined by counting NBT positive cells and inhibited proliferation of HL-60 human leukemic cells in dose dependent manner (IC50 = 4 mM). At a bpV dose of 1.3 mM 50% of the cells differentiated. After 6 days of incubation at a bpV dose of 3 mM 82.1% of cells were differentiated. Under our culture conditions the doubling time of HL-60 cells was 30.7 h; addition of bpV (3 mM) increased doubling time to 48 h. In rat hepatoma 3924A cells bpV inhibited proliferation in a time- and dose- dependent fashion IC50 = 0.5 mM after 72 h of incubation. bpV and quercetin in combination are additivelly cytotoxic in hepatoma 3924A cells.
bisperoxovanadate-1;10-phenantroline; phosphotyrosine phosphatase inhibitor; HL60 cell differentiation
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nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
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Podaci o prilogu
128-129-x.
1997.
objavljeno
Podaci o matičnoj publikaciji
Šesti kongres biologa Hrvatske
Hrvatsko biološko društvo
Hrvatsko biološko društvo
Podaci o skupu
Šesti Kongres Biologa Hrvatske
poster
22.09.1997-26.09.1997
Opatija, Hrvatska