engleski

In vitro propagation of Fibigia triquetra (DC.) Boiss., a rare stenoendemic species

A rapid clonal propagation method for Fibigia triquetra (DC.) Boiss. (Brassicaceae), a rare Croatian stenoendemic species, has been developed. Shoots originated from aseptically germinated seeds were used for culture initiation. The highest multiplication rate of 9.2 shoots per explant was achieved in 4 weeks culture period on half strength Murashige and Skoog medium supplemented with 0.5 ľM 6-benzylaminopurine and 2.9 ľM gibberellic acid. Excised shoots were rooted on the same basal medium with addition of 8.6 mM indole-3-butyric acid. Rooted plantlets were successfully transferred to potting soil and acclimatized to outdoor conditions.

endemic plant; Fibigia triquetra (DC.) Boiss.; micropropagation

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