engleski

Carbonic anhydrase activity on total esterase activity in Mediterranean mussel Mytilus galloprovincialis

The esterase activity of carbonic anhydrase (CA) was investigated in mussels sampled at 19 locations along the Croatian coast of Adriatic Sea. The gills were the target tissue, because the respiratory, ionic transport and pH regulatory enzyme function of CA and its potential usage as biomarkers of environmental pollution was the main topic. So far no report has dealt with the esterase activity of CA on total esterase activity level in mussel. Previously, the possible application of CA activity inhibition as an in vitro bioassay for detection of heavy metals in pollution monitoring using filter-feeding mussel Mytilus galloprovincialis was suggested. Total esterase activity was measured in cytosolic fraction by colorimetric end-point reaction using p-nitrophenyl acetate as enzyme substrate. CA activity was estimated by the same enzymatic reaction using acetazolamide as a specific CA inhibitor. The results of total esterase activities in winter (March ; average value 0.137±0.057) were lower then determined for summer season (August ; 0.153±0.036) at almost all investigated locations. CA activities determined in gills of mussel sampled in winter ranged from 1.75 % to 24.65 % of total esterase activity. CA activities in summer samples were practically non detectable. With this poster we report a cDNA CAII sequence of M. galloprovincialis which is according to the NCBI database, the first normal one for Mollusca, Bivalvia, besides Nacrein and nacrein-like proteins. In spite of in vitro studies showing clear inhibition of CA activity with heavy metals, for the application of CA as a biomarker of pollution further research on the environmental and physiological factors effect, different CA isozymes characterization and sequencing are needed.

Mytilus galloprovincialis; carbonic anhydrase; total esterase activity; CAII cDNA sequence

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