Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4- dichloroisocoumarin (CROSBI ID 175268)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Leščić Ašler, Ivana ; Pigac, Jasenka ; Vujaklija, Dušica ; Luić, Marija ; Štefanić, Zoran
engleski
Crystallization and preliminary X-ray diffraction studies of a complex of extracellular lipase from Streptomyces rimosus with the inhibitor 3, 4- dichloroisocoumarin
A recombinant lipase (triacylglycerol acylhydrolase ; EC 3.1.1.3) from the bacterium Streptomyces rimosus was inhibited by the serine protease inhibitor 3, 4-dichloroisocoumarin and crystallized by the hanging-drop vapour-diffusion method at 291 K. The crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 38.1, b = 78.7, c = 56.6 Å, [beta] = 104.5° and probably two molecules in the asymmetric unit. Diffraction data were collected to 1.7 Å resolution using synchrotron radiation on the XRD beamline of the Elettra synchrotron, Trieste, Italy.
streptomyces rimosus ; SGNH hydrolases ; lipases ; 3 ; 4-dichloroisocoumarin
f logocrystallization communications share
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Podaci o izdanju
67 (11)
2011.
1378-1381
objavljeno
1744-3091
10.1107/S1744309111032222