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Blockade of phrenic long term facilitation by microinjections of the 5-HT1A receptor antagonist WAY-100635 into the raphe nucleus of the rat (CROSBI ID 578162)

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Pavlinac, Ivana ; Pecotic, Renata ; Đogaš, Zoran ; Valić, Maja Blockade of phrenic long term facilitation by microinjections of the 5-HT1A receptor antagonist WAY-100635 into the raphe nucleus of the rat // International Brain Research Organisation. 2011

Podaci o odgovornosti

Pavlinac, Ivana ; Pecotic, Renata ; Đogaš, Zoran ; Valić, Maja

engleski

Blockade of phrenic long term facilitation by microinjections of the 5-HT1A receptor antagonist WAY-100635 into the raphe nucleus of the rat

Sustained augmentation of phrenic nerve activity evoked by acute intermittent hypoxia (AIH) represents form of central nervous system plasticity, known as phrenic long term facilitation (pLTF). Previous studies have shown that pLTF is serotonin (5-HT) dependent, and systemic application of selective and non- selective 5-HT receptor antagonists could impair induction and preservation of pLTF. Microinjections of non-selective 5-HT receptor antagonist methysergide into the raphe nuclei prevented identification of specific 5-HT receptor subtype involved in modulation of pLTF at supraspinal level. Therefore, present study was preformed to determine whether blockade of 5-HT1A receptors in caudal raphe region influences induction of pLTF. Our hypothesis was that microinjections of selective 5-HT1A receptor antagonist WAY- 100635 into the caudal raphe nucleus could prevent induction of phrenic LTF after exposure to AIH. Adult, male, urethane- anesthetized, vagotomized, paralyzed and mechanically ventilated Sprague-Dawley rats were exposed to AIH protocol. Experimental group of animals (n=7) received microinjection of WAY- 100635 (1mM, 20±5 nl) into the caudal raphe nucleus, whereas control group (n=7) received microinjection of 0.9% saline (20±5 nl) into the same site. Peak phrenic nerve activity (pPNA), burst frequency (f), and respiratory rhythm parameters were analyzed during five hypoxic episodes (TH1-5), as well as at 15 (T15), 30 (T30), and 60 (T60) minutes after the end of the last hypoxic episode. In the control group, pPNA was elevated from baseline (to 172.9±17.5%, P=0.008) at 60 minutes after episodic hypoxia, indicating pLTF. Microinjections of WAY-100635 into the raphe nuclei prior to hypoxic stimulation attenuated pLTF. There was no significant decrease of pPNA from baseline (-15.4±16.9%) at T60. Microinjections of selective 5-HT1A receptor antagonist WAY-100635 into the raphe nuclei prior to episodic hypoxia exposure prevented induction of pLTF, indicating that 5-HT1A receptor activation at supraspinal level is important for induction of pLTF.

phrenic nerve; intermittent hypoxia; long term facilitation; raphe nuclei; serotonin

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Podaci o prilogu

2011.

nije evidentirano

objavljeno

Podaci o matičnoj publikaciji

International Brain Research Organisation

Podaci o skupu

World Congress of Neuroscience

poster

14.06.2011-18.06.2011

Firenca, Italija

Povezanost rada

Temeljne medicinske znanosti