Application of Chip-Based Nanoelectrospray Ion Trap Mass Spectrometry to Compositional and Structural Analysis of Gangliosides in Human Fetal Cerebellum (CROSBI ID 176673)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Mosoarca, Cristina ; Ghiulai, Roxana M. ; Novaconi, Cristina R. ; Vukelić, Željka ; Chiriac, Adrian ; Zamfir, Alina D.
engleski
Application of Chip-Based Nanoelectrospray Ion Trap Mass Spectrometry to Compositional and Structural Analysis of Gangliosides in Human Fetal Cerebellum
Two native ganglioside mixtures from normal human fetal cerebellum in the 15th (Cc15)and 40th (Cc40) gestational week were subjected to NanoMate high capacity ion trap (HCT) mass spectrometric (MS) and collision induced dissociation (CID) tandem MS (MS2)analysis under thoroughly optimized experimental conditionns. An total of 56 different species were identified in Cc15 and 54 in Cc40. By employing CID MS2 molecular ions, related GD1 (d18:1/20:0) and GM2 (d18:1/19:0) species were structurally characterized in a high throughput mode. The method provided elevated ionization efficiency, high speed of analysis, almost 100% reproducibility at sample consumption per experiment situated in the femtomole range.
automation; cerebellum; chip-nanoelectrospray; gangliosides; ion trap mass spectrometry; NanoMate
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Podaci o izdanju
Povezanost rada
Temeljne medicinske znanosti
