Sgs1 controls the fidelity of gene targeting (CROSBI ID 579073)
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Podaci o odgovornosti
Štafa, Anamarija ; Miklenić, Marina ; Lisnić, Berislav ; Svetec, Ivan-Krešimir ; Zgaga, Zoran
engleski
Sgs1 controls the fidelity of gene targeting
Introduction of linear, non replicative DNA fragments is widely used for the replacement of homologous sequences in the yeast genome. Moreover, it is studied as a general model for genetic manipulations by ends-out recombination. It is generally assumed that, in contrast to higher eukaryotes, transformation of the yeast Saccharomyces cerevisiae with non replicative DNA occurs almost exclusively by homologous recombination. We found out that the ends-out recombination may result in different genetic alterations in the yeast genome (1). Three clases of events were identified: illegitimate integration, integration of the transforming DNA next to the targeted region and disomic, heteroallelic transformants containing starting allele and allele expected after succesful gene targeting. We performed a systematic study of the influence of type of gene modification (insertion, replacement and deletion) on the accuracy of ends-out recombination.
ends-out recombination; chromosome duplication; Saccharomyces cerevisiae
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Podaci o prilogu
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Podaci o skupu
Genome Integrity Discussion Group, The New York Academy of Sciences, Petrini J., Rothstein R., Symington L.S. ur, New York, SAD
poster
06.06.2011-06.06.2011
Sjedinjene Američke Države