engleski

Determination of 3J(1H3′-31P) couplings in a DNA oligomer with enhanced sensitivity employing a constant-time TOCSY difference experiment

A constant-time TOCSY difference experiment for the determination of 3J(1H3′-31P) coupling constants in non-isotope-labelled DNA oligonucleotides is presented. The method is tested on a DNA octamer and compared with the established constant-time NOESY difference method. Each 3J(1H3′-31P) coupling constant is determined from amplitude changes caused by phosphorous decoupling, which are observable on multiple cross-peaks, thus leading to a high accuracy of the value of the 3J(1H3′-31P) coupling constant. The new experiment delivers up to three times the sensitivity compared with previously reported methods.

NMR ; constant-time TOCSY experiment ; DNA oligomer ; 3J(1H3′-31P) coupling constants

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