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Mathematical modelling of bioethanol production from raw sugar beet juice

Ethanol as biofuel can significantly reduce the negative environmental impacts of fossil fuels. However, bioethanol production from renewable resources is still more expensive than gasoline production from fossil oil. Integrated bioprocess combines different steps in bioethanol production (fermentation, separation) in one single unit thus making it more cost-effective and energy saving. Different cultivation techniques, weather using liquid or solid substrate, can significantly increase ethanol yield and consequently improve the performance of the process. Production of ethanol on the raw sugar beet juice was studied. For ethanol production on the raw sugar beet juice batch and fed batch cultivation techniques were used during research in the stirred tank bioreactor. Saccharomyces cerevisiae was used as a working microorganism. All concentrations (supstrate and products) were measured using HPLC. Experimental data served for the development of mathematical models describing supstrate utilization and product and biomass formation via computational program Madonna. During batch ethanol production on the raw sugar beet juice ethanol yield was 59.89 g/L and efficiency 78.8 %. In fed batch process ethanol yield was 92.78 g/L and efficiency 93.4 %, respectively. Unstructured kinetic models correspond well with obtained experimental data. Experimental results suggest that sugar beet juice can be successfully used for ethanol production.

bioethanol; mathematical modelling; unstructured kinetic models

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