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Clinical and molecular characterization of five patients with succinyl-CoA:3-ketoacid CoA transferase (SCOT) deficiency (CROSBI ID 178057)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Fukao, Toshiyuki ; Sass, Joern Oliver ; Kursula, Petri ; Thimm, Eva ; Wendel, Udo ; Ficicioglu, Can ; Monastiri, Kamel ; Guffon, Nathalie ; Baric, Ivo ; Zabot, Marie-Therese et al. Clinical and molecular characterization of five patients with succinyl-CoA:3-ketoacid CoA transferase (SCOT) deficiency // Biochimica et biophysica acta, 1812 (2011), 5; 619-624. doi: 10.1016/j.bbadis.2011.01.015

Podaci o odgovornosti

Fukao, Toshiyuki ; Sass, Joern Oliver ; Kursula, Petri ; Thimm, Eva ; Wendel, Udo ; Ficicioglu, Can ; Monastiri, Kamel ; Guffon, Nathalie ; Baric, Ivo ; Zabot, Marie-Therese ; Kondo, Naomi

engleski

Clinical and molecular characterization of five patients with succinyl-CoA:3-ketoacid CoA transferase (SCOT) deficiency

Succinyl-CoA:3-ketoacid CoA transferase (SCOT) deficiency is an inborn error of ketone body metabolism and causes episodic ketoacidosis. We report clinical and molecular analyses of 5 patients with SCOT deficiency. Patients GS07, GS13, and GS14 are homozygotes of S405P, L327P, and R468C, respectively. GS17 and GS18 are compound heterozygotes for S226N and A215V, and V404F and E273X, respectively. These mutations have not been reported previously. Missense mutations were further characterized by transient expression analysis of mutant cDNAs. Among 6 missense mutations, mutants L327P, R468C, and A215V retained some residual activities and their mutant proteins were detected in immunoblot analysis following expression at 37°C. They were more stable at 30°C than 37°C, indicating their temperature sensitive character. The R468C mutant is a distinct temperature sensitive mutant which retained 12% and 51% of wild-type residual activities at 37 and 30°C, respectively. The S226N mutant protein was detected but retained no residual activity. Effects of missense mutations were predicted from the tertiary structure of the SCOT molecule. Main effects of these mutations were destabilization of SCOT molecules, and some of them also affected catalytic activity. Among 5 patients, GS07 and GS18 had null mutations in both alleles and the other three patients retained some residual SCOT activities. All 5 developed a first severe ketoacidotic crisis with blood gas pH <7.1, and experienced multiple ketoacidotic decompensations (two of them had seven such episodes). In general, the outcome was good even following multiple ketoacidotic events. Permanent ketosis or ketonuria is considered a pathognomonic feature of SCOT deficiency. However, this condition depends not only on residual activity but also on environmental factors.

Succinyl-CoA:3-ketoacid CoA transferase (SCOT) deficiency

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nije evidentirano

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Podaci o izdanju

1812 (5)

2011.

619-624

objavljeno

0006-3002

10.1016/j.bbadis.2011.01.015

Povezanost rada

Kliničke medicinske znanosti

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