Fas deficiency attenuates bone loss during antigen induced arthritis in mice (CROSBI ID 580805)
Prilog sa skupa u časopisu | sažetak izlaganja sa skupa | međunarodna recenzija
Podaci o odgovornosti
Lazić Mosler, Elvira ; Kuzmac, Sania ; Ivčević, Sanja ; Grčević, Danka ; Marušić, Ana ; Kovačić, Nataša
engleski
Fas deficiency attenuates bone loss during antigen induced arthritis in mice
Background Antigen induced arthritis (AIA) is an experimental model of rheumatoid arthritis induced by methylated bovine serum albumin (mBSA) [1]. Hyperplastic synovia in AIA contains fibroblast- like synoviocytes (FLS) with reduced ability to differentiate into osteoblasts, chondroblasts or adipocytes [2]. Since Fas is shown to inhibit osteoblast differentiation [3], we were interested whether such inhibitory effect may contribute to the pathogenesis of AIA. Materials and methods AIA was induced in mice with knocked-out Fas gene (Fas –/–). Three weeks after pre-immunization with mBSA in complete Freund's adjuvant, wild-type (C57BL/6, wt) and Fas –/– mice were injected with mBSA into each knee, whereas controls were injected with equal volume of phosphate buffered saline (PBS). Three weeks after injection we assessed joint diameters, histology, μCT scans, and differentiation of bone marrow- and synovia- derived osteoblasts. Results Knee diameters were increased in mBSA-injected wt mice compared to PBS-injected controls (3.21±0.2 vs. 2.98±0.1, p<0.05, t-test), and this increase was not significant in Fas –/– mice (2.97±0.2 vs. 2.87±0.1). Histology revealed presence of synovial hyperplasia in both mBSA-injected groups, but mBSA-injected wt mice had decreased trabecular bone volume in distal femoral metaphyses (BV/TV) compared to controls (1.08±0.57 vs. 2.55±0.43 ; p<0.05, t-test). There was no significant difference between mBSA-injected and control group in Fas –/– mice (2.34±0.62 vs. 2.61±0.65). μCT analysis showed that mBSA-injected wt mice had decreased BV/TV (2.99±0.19 v. 1.96±0.19 ; p<0.001, t-test) and trabecular number (TbN) (1.03±0.03 vs. 0.64±0.02), as well as increased trabecular separation (TbSep) (256, 89±1395, 12 vs. 312.40±1323.91), compared to controls. mBSA injected Fas –/– mice had deacresed TbN compared to controls (0.815±0.01 vs. 0.64±0.04 ; p<0.05, t- test), with no significant difference in other trabecular parameters. Osteoblast differentiation was increased in both wt and Fas –/– mBSA-injected mice. Conclusions Our study revealed that Fas deficiency attenuated clinical development and bone loss in AIA, and mechanism of this phenomenon needs to be clarified.
arthritis; osteoblasts; CD95; bone loss
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Podaci o prilogu
2011.
nije evidentirano
objavljeno
Podaci o matičnoj publikaciji
Arthritis research & therapy
London : Delhi: BioMed Central
1478-6354
1478-6362
Podaci o skupu
1st Bio-Rheumatology International Congress (BRIC2011)
poster
14.11.2011-16.11.2011
Tokyo, Japan
Povezanost rada
nije evidentirano