engleski

The phenanthridine biguanides efficiently differentiate between dGdC, dAdT and rArU sequences by two independent, sensitive spectroscopic methods

At submicromolar concentrations two novel phenanthridine biguanides efficiently differentiate between dGdC and dAdT sequences by opposite fluorimetric changes (quenching for dGdC and increase for dAdT) and especially bis- biguanide derivative gives opposite ICD response (negative ICD for dGC and strong positive for dAdT). This specific signalling was explained by ability of compounds to switch binding mode from intercalation into dGdC to minor groove binding into dAdT sequences. Both compounds bind to rArU by intercalation, yielding different fluorimetric and CD response in comparison to any ds-DNA. Moreover, both compounds revealed significantly higher affinity toward ds-polynucleotides in comparison to previously studied alkylamine- and urea-analogues.

phenanthridine; DNA; RNA; fluorescence; CD

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