engleski

MOLECULAR IDENTIFICATION OF RAINBOW TROUT SLEEPING DISEASE VIRUS IN CROATIA

Sleeping disease virus (SDV) is a member of salmonid alphaviruses and is recognized as a serious pathogen of farmed rainbow trouts in Europe. It affects trouts at all stages of production with variable mortality levels. The present study reports molecular identification of sleeping disease virus occurred at one salmonid fish farm in Croatia. RT-PCR (reverse transcription-polymerase chain reaction) was applied for SDV detection in moribund, 10-month-old rainbow trouts. Two products, partial E2 and nsP3 gene were amplified and sequenced. Obtained sequences confirmed SDV presence and revealed close genetic similarity with previously reported SDV sequences found in database. Finding of SDV in Croatia suggests import of this virus through the fish trade and indicates significance of regular fish health monitoring.

rainbow trout; sleeping disease virus; Croatia

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