engleski

Paraoxonase-1 activity in patients with angiographically assessed peripheral arterial disease.

Background: Low serum paraoxonase 1 (PON1) activity is a biochemical risk factor for atherosclerotic disease. Aim: of the study is to compare the serum PON1 activity and HDL cholesterol (HDL-C) concentration in patients with angiographically assessed obliteration or peripheral arterial stenosis of different degrees and matched control subjects. Patients and Methods: The venous blood samples were collected by venipuncture from 133 healthy individuals (median age 58 years, range 43−82) and 203 patients with symptomatic peripheral artery disease (median age 64 years, range 40−88), with peripheral arteries obliteration (N = 164) or >50% of peripheral arteries stenosis (N = 39), assessed angiographically. Paraoxon was used as a substrate for measuring basal or sodium chloride (NaCl)- stimulated PON1 activity. For HDL-C concentration determination direct method based on selective inhibition of the non-HDL fraction was used. The Man-Whitney U test was applied to evaluate the difference between the groups (p < 0.05 was considered statistically significant). Results: Compared to control subjects basal (median values 87 vs. 130 U/L ; range 17–495 vs. 46–670 U/L) and stimulated (median values 170 vs. 248 U/L ; range 36–984 vs. 90–1184 U/L) PON1 activity and HDL-C concentration (median value 1.1 vs. 1.6 mmol/L ; range 0.6−2.4 vs. 0.8.-3.4 mmol/L) were significantly lower in patients with angiography assessed stenosis >50% or obliterations. There were no statistically significant difference between these two group of patients. Conclusion: The obtained results suggest that lower serum basal and stimulated PON1 activity, as well as HDL-C concentration could be used as biochemical risk factors for peripheral arterial disease.

serum paraoxonase 1 (PON1); HDL cholesterol; angiographically assessed obliteration or peripheral arterial stenosis

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