engleski

Antimutagenic effect of indole glucosinolates from Cruciferae family on 1-aminoanthracene-induced mutagenicity in prokaryotic test-sistem

Glucosinolates are secondary plant metabolites widely distributed in plants of Cruciferae family (e.g.) Brussels sprouts, broccoli, cauliflower, cabbage). It has been shown that these natural plant compounds can be easily hydrolyzed to produce a range of physiologically active agents (isothiocyanates, nitriles and thiocyanates), some of them with a significant antimutagenic/anticarcinogenic effect. The aim of our invesitgation was to examine cytotoxic, mutagenic and antimutagenic effect of an extract of glucosinolates from cauliflower (CY), which endogenous myrosinase normally generates by hydrolysis of 3-indolylmethyl glucosinolate. To investigate the potential cytotoxic, mutagenic and antimutagenic effects of this extract, we used sereval different test-protocols suggested by modified Ames test, DeFlora, Dashwood and Preobrazhenskaya on prokaryotic test-system, bacteria Salmonella typhimurium strain TA98 with metabolic activation. According to cytotoxic examination, the results showed that CY caused a weak cytotoxic effect, but only at the highest concentration. For this reason our further mutagenic and antimutagenic investigations were based on cleary, noncytotoxic doses, where the survivals of bacteria were up to 90%. So, the obtained data showed that CY had no mutagenic effect at any applied doses. On the other side, this glucosinolates-rich extract successfully inhibit the action of 2-aminoanthracene (2-AA). On the end, we strongly suggest that investigations on possible inhibition of the tumor cell growth in presence of CY, and consequently its anticarcinogen effect should be made.

antimutagenic effect; glucosinolates; prokaryotic test-system

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