engleski

BOTULINUM TOXIN A IN EXPERIMENTAL MIGRAINE

Introduction/Objectives: Control studies in humans indicate that botulinum toxin type A (BoNT/A) alleviates symptoms of migraine. However, this problem was not investigated in details in experimental animals. Infraorbital nerve constriction injury is a model used both in trigeminal neuropatic pain and in migraine. We have assessed mechanical allodynia and dural extravasation in this model after single unilateral injection of BoNT/A (3.5 U/kg). Participants, Materials/Methods: Neuropathic behavior in rats was induced by a chronic constriction injury of the infraorbital nerve, and BoNT/A was administered into whisker pad either ipsilaterally or contralaterally to the side of nerve injury. Th e effects of BoNT/A on bilateral pain were assessed for a period of a month by measuring mechanical allodynia with Von Frey fi laments. Animals which developed mechanical allodynia were administered with Evansblue intravenously and dural extravasation of Evans blue - plasma protein complexes was assessed. Results: Both mechanical allodynia and dural extravasation were reduced by a single unilateral BoNT/A (3.5 U/kg) injection on the side of the toxin injection, and on the opposite side, as well. Conclusions: Both migraine signs: pain and dural extravasation were reduced after BoNT/A injection. For the fi rst time we show presence of dural extravasation after infraorbital nerve constriction injury. Bilateral antinociceptive eff ect and even more reduction of dural extravasation suggests a central mechanism of BoNT/A actions.

migrena; dura; ekstravazacija

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