engleski

Genetic identification of 'Bistrica' and her synonyms 'Požegača' and 'Hauszwetsche' (Prunus domestica L.) using SSRs

'Bistrica' is well known and widespread plum variety in the area of former Austro-Hungarian Empire being under cultivation for more than 300 years. Although 'Bistrica' is well known and described variety, due to often observed phenotypic variation it has been reported as “landrace cultivar” consisted out of many different “biotypes”. Thus, reliable genotype identification in situ in old traditional orchards can be difficult. The aim of this study was to determine its SSR profile and to estimate its genotype homogeneity across the region. Therefore 33 accessions of 'Bistrica' and her synonyms were sampled in both traditional orchards and nurseries from Bosnia & Herzegowina, Croatia and Serbia. A known synonym cultivar, originating from Austria ('Hauszwetsche'), was used as a referent genotype too. Set of 11 highly polymorphic SSR markers was used for molecular characterization and PCR products were detected using ABI 3130 Genetic Analyzer (Applied Biosystems). Three accessions out of 33 had a genotype different at most of the analyzed loci and we excluded them from further consideration as homonyms. Nineteen out of 33 accessions shared identical genotype at 11 SSR loci having 4-6 alleles per locus. The origin of these accessions was partly from nurseries (10 samples from three different nurseries in Croatia and Serbia) and partly from traditional orchards of rather distant regions of Bosnia & Herzegovina and Croatia, including the sample from Austria. We concluded this SSR profile might be the basic one for 'Bistrica'. After several repetition of whole genotyping work we confirmed that the remaining 11 accessions have slightly different genotype to the basic one, mostly at a single locus (9 accessions) while two accessions had different alleles at two and three loci. Considering the complexity of plum hexaploid genome and number and gene diversity of applied SSR loci we assume these accessions have the same unizygote origin as previous 19 ones, and observed variation might be the result of spontaneous mutations.

plum ; molecular characterization ; genotype homogeneity ; traditional orchards ; nurseries

Rad je prezentiran na skupu 13th Eucarpia Symposium on Fruit Breeding and Genetics.