Evaluation of a molecular fingerprinting method based on the diversity of the ribosomal 16S rRNA gene to describe the dynamic of bacterial communities in Istrian cheese (CROSBI ID 179498)
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Mrkonjić Fuka, Mirna ; Radl, Viviane ; Bogovič Matijašić, Bojana ; Schloter, Michael
engleski
Evaluation of a molecular fingerprinting method based on the diversity of the ribosomal 16S rRNA gene to describe the dynamic of bacterial communities in Istrian cheese
Denaturing gradient gel electrophoresis (DGGE) is a powerful tool to study bacterial communities’ structure based on the diversity of the 16S rRNA genes. However, the results are strongly dependent on different steps included into the method. Therefore, three primer pairs that amplify different variable regions of the 16S rRNA gene (V1, V3 and V6- V8) were tested in order to investigate the bacterial diversity existent in Istrian cheese. We found that primer set extremely influenced DGGE analysis. V3 primers were most efficient when 15 cheese associated isolates were resolved by DGGE. However, for Istrian cheese analysis the best separation and highest number of bands in DGGE patterns were noticed for V6-V8 primer pairs.
DGGE; 16S rRNA gene; cheese
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