engleski

Bovine embryo production in vitro by Ovum Pick-Up – an overwiev of 10 years of investigation in Croatia

Ultrasound-guided ovum pick-up (OPU) followed by in vitro embryo production offers an alternative to produce offspring from genetically superior dams, pregnant cows and heifers, post-partum cows and even from prepubertal heifers and cows with reproductive disorders. Over the last decade basic and applied research projects have been conducted in the Laboratory for Assisted Reproduction of Faculty of Veterinary Medicine in Zagreb. A present standard protocol for IVP of bovine embryos is based on the use of BoviPure® for sperm preparation for in vitro fertilization (IVF) and synthetic oviductal fluid with amino acids and bovine serume albumine (SOFaaBSA) for in vitro culture (IVC). During the 10 years period more than 230 OPU sessions were carried out in 63 stimulated and/or cyclic heifers and cows, resulted with 578 transferable embryos. Different synchronization treatments for OPU/IVP donor heifers were investigated: prostaglandin F2 administration without or with dominant follicle removal (DFR) and norgestomet ear implant insertion with DFR. The OPU/IVF results were compared in PMSG and pFSH stimulated donors with or without DFR, as well in cyclic donors when OPU was performed twice a week during a month period. Follicular dynamics and in vitro embryo production after repeated superovulation with pFSH in Simmental heifers were monitored, as well the effect of the follicle size on developmental competence of bovine oocytes. The effect of the origin of the immature oocytes (slaughterhouse or OPU), a well the effect of donor breed (Charolais, Simmental and Holstein.Frieian) for OPU/IVP were compared. Transvaginal OPU/IVP was also performed in problem cows, suffering from cystic ovaries or post-partum anestria due to endometritis with satisfactory results. Using OPU/IVP good quality bovine blastocysts were produced in vitro suitable for embryo transfer or cryopreservation, comparable to other laboratories for IVP of bovine embryos.

cow; oocyte; embryo; ovum pick-up; fertilization in vitro

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