engleski

Antimutagenic effectrs of Brassica vegetables on bacterial test systems and human cell lines

Glucosinolates are a group of compounds widely distributed among the plants of Cruciferae family (e.g., a broccoli, cauliflowers, cabbage). These compounds are easily hydrolyzed by the enzyme myrozinase wich becomes activated after mechanical destruction of the plant cell. Isothiocyanates, nitriles, thiocyanates and indole compounds are most common products of such an hydrolysis. Indole compounds affect some mutagens. Our earlier results have shown that indole glucosinolates and indole-3-carbinol have neither mutagenic nor cytotoxic effect. The aim of this study was to investigate potential antimutagenic effect of cauliflower extract (CY) and indole-3-carbinol (I3C) against promutagen 2-aminoanthracene on bacterial test strains Salmonella typhimurium TA98 and TA100. The best results were obtained when cells were treated according to protocol for simultaneous treatment. Also, the influence of CY and I3C on the intracellular level of glutathione (GSH) and activity of glutathione transferases (GST) were examinated on parental laryngeal carcinoma cells (HEp2) and their cisplatin resistant subline (CK2). The results show that both, CY and I3C increased the level of GSH in parental cells to the similar level. On the contrary, in CK2 cells, which have increased basal level of GSH, both drugs decreased the level of GSH. CY was in this respect more efficient. The activity of GSTs were increased in parental cells after treatment with CY or with I3C. On the other hand, CY caused significant decrease of GST activity in CK2 cells.

glucosinolates; antimutegenic effect; Salmonella typhimurium; laryngeal carcinoma cells

nije evidentirano