engleski

Incidence and growth of methylcholantrene-induced tumors in mice with altered immunological status

BALB/c mice were treted s.c. with 3-methylcholanthrene and tumor incidence and growth were followed for 9 months.Immunological status of mice was altered by various treatments. Thymectomized, lethally irradiated, bone marrow reconstituteed mice served as T-cell deficient recipients. In order to suppres natural killer-cell/macrophage functions some mice were injected with silica particles ; to enhance these functions some mice were given Corynebacterium parvum. Silica and CP were given simultaneously with MCA to test their influence on the presumed function of surveillance of tumor incidence, and also 2 months after MCA to test their influence on the growth of grater numbers of transformed host cells. Almost all mice developed tumors at the inoculation site and at the end of the observation period there was no difference in tumor incidence among 9 experimental groups. However, in T-cell deficient mice we observed shorter tumor duration and earlier death than in normal mice. Silica particles appeared to enhance tumor growth but the differences compared to normal controls were not significant. A single injection of CP simultaneously with MCA caused earlier tumor appearance but also slowed its growth. In contrast, CP given 2 months after MCA significantly delayed the appearance of the tumors. In regard to the tumor growth immunosuppression had stronger effects in males than in females ; the opposite was true for immunostimulation treatments. We concluded that immunological status does not influence long-term tumor incidence but that both T-cell and NK-cell/macrophage compartments strongly influence the parameters of growth of chemically induced tumors, i.e., the immune and natural resistance mechanisms do not influence thefrequency of de novo arising tumors but that both can slow down tumor growth.

incidence of methylcholantrene-induced tumors; growth; mice; altered immunologcal status

nije evidentirano