engleski

Biological repair of thyroid cartilage defects by osteogenic protein-1 (BMP-7) and different carriers in dog

We have recently shown that OP-1 bound to thyroid alograft regenerated large thyroid cartilage defects by inducing new bone, cartilage and ligament-like structures (Katic et al, Growth Factors 16, 1999). In the present study we tested the efficacy of OP-1 in regeneration of thyroid cartilage defects (2cm2) in dog using different carriers. OP-1 with a carrier was applied to the defect area and maintained between perichondrial layers adjacent to the defect. The treatment groups (n=4) were as follows:I/OP-1/CMC device, II/OP-1/CMC/blood and III/OP-1/Helistat sponge. Dogs were killed four months following surgery. Each larynx was removed, carefully dissected and three-dimensinal reconstruction of defect area was performed on serial sections. The results revealed that thyroid defects in group I healed almost completely by newly formed bone and ligament. New bone was well connected to both cartilage ends. This is the first evidence that CMC can serve as a carrier for recombinant BMP. Defects in group II and III also healed completely by bone and ligament tissue in a very remodelinf phase, but no apparent cartilage was evident. This study suggests that in the presence of different carriers, where tissue processing is not controlled by slow release of morphogen and guidance by an extracellular matrix carrier, osteogenesis prevails above chondrogenesis in regenerating thyroid cartilage defects.

Bone morphogenetic protein; thyroid cartilage; larynx recontruction

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