engleski

Phylogenomic profile of DNA recombinational repair

DNA repair is an essential mechanism for preservation of genome stability. Since double-strand breaks are especially hazardous to living organism, the machinery involved in repair of these lesions is conserved and ubiquitous in all organisms that favor the integrity of genomic information. Although double-strand breaks in DNA can be repaired through non-homologous end joining, the recombinational repair is more reliable, accurate and therefore crucial for maintenance of genomic information. A detailed analysis of proteins involved in DNA recombinational repair (RER) was performed by comparing the ORF sequences of recombinational repair proteins from model organism yeast Saccharomyces cerevisiae to entire set of protein sequences encoded in completely sequenced genomes of Archaea, Bacteria and Eukarya. A set of 20 proteins involved in RER in yeast was chosen based on literature and annotation information (mainly the RAD52 epistasis group), as well as from recent data originating from large-scale protein complex analysis of yeast cell machinery. We attempt to correlate presence or absence of certain repair proteins to environmental conditions and life style of particular systematic group of organisms and create a comprehensive phylogeny of recombinational repair in all three domains of life.

phylogenomic profile; recombinational repair

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