engleski

Repetitive sequences in Eurasian lynx (Lynx lynx L.) mitochondrial DNA control region

Mitochondrial DNA (mtDNA) control region (CR) of numerous species is known to include up to five different repetitive sequences (RS1–RS5) that are found at various locations, involving motifs of different length and extensive length heteroplasmy. Two repetitive sequences (RS2 and RS3) on opposite sides of mtDNA central conserved region have been described in domestic cat (Felis catus) and some other felid species. However, the presence of repetitive sequence RS3 has not been detected in Eurasian lynx (Lynx lynx) yet. We analyzed mtDNA CR of 35 Eurasian lynx (L. lynx L.) samples to characterize repetitive sequences and to compare them with those found in other felid species. We confirmed the presence of 80 base pairs (bp) repetitive sequence (RS2) at the 50 end of the Eurasian lynx mtDNA CR L strand and for the first time we described RS3 repetitive sequence at its 30 end, consisting of an array of tandem repeats five to ten bp long.We found that felid species share similar RS3 repetitive pattern and fundamental repeat motif TACAC.

Eurasian lynx; Lynx lynx; mtDNA; control region; repetitive sequences

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