Effect of Th1 type cytokines (IL-2, IL-12, IL-15) on cytotoxic activity of decidual NK cells (CROSBI ID 477900)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | domaća recenzija
Podaci o odgovornosti
Štrbo, Nataša ; Laškarin, Gordana ; Sotošek, Vlatka ; Bogović, Tatjana ; Randić, Ljiljana ; Podack, Eckhard R. ; Rukavina, Daniel
engleski
Effect of Th1 type cytokines (IL-2, IL-12, IL-15) on cytotoxic activity of decidual NK cells
It has been suggested that decidual NK cells (dNK) besides functions in the defence against virally infected and transformed cells may contribute also to the control of cytotrophoblast invasion of the placental bed during early pregnancy. For these functions dNK cells are abundantly endowed with the perforin (Pbright), cytolytic mediator at the molecular level. Pregnancy has been defined as a Th2 phenomenon, with a prevalence of Th2 type cytokines at the maternal-fetal interface. Since cytolytic activity is regulated primarily by Th1 type cytokines, and suppressed by Th2 type cytokines, the question is which cytokines at the interface have a dominant role. IL-2 has not been detected in the normal implantation site, but IL-15 which shares many biological properties with IL-2 has been abundantly expressed in the placenta. IL-12 is expressed in placenta in small quantities and it is a cytokine capable of inducing differentiation of naive T cells to Th1 phenotype. The aim of this study was to investigate the role of Th1 cytokines (IL-2, IL-12, IL-15) in the control of NK cytotoxicity of decidual lymphocytes (DL). Cytolytic activity of unstimulated and Th1 cytokine stimulated dNK cells was tested against K562 cell line in a 2 hr cytotoxicity PKH 26 (red) test. The frequency of P+ cells and P content per cell were measured simultaneously. Cytolytic potential of DL is highly increased by IL-2 (1000 i.u./ml) and IL-12, but surprisingly suppressed by IL-15. This suppression is released in the combination of other Th1 type cytokines. These results are presenting that P mediated cytotoxicity under the influence of Th1 type cytokines might have an important role in the protection of the feto-placental unit against both maternally acquired and local infections at the interface. These investigations are financially supported by the Ministry of Science and Technology of the Republic of Croatia (Grant No. 062001).
Th1 cytokines; decidual NK cells; cytotoxic activity
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Podaci o prilogu
SOP4-SOP4.
1999.
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objavljeno
Podaci o matičnoj publikaciji
Annual meeting of the Croatian Immunological Society 1999 : Abstract book
Rabatić, S.
Zagreb: Hrvatsko imunološko društvo
Podaci o skupu
Annual meeting of the Croatian Immunological Society 1999
predavanje
25.11.1999-25.11.1999
Zagreb, Hrvatska