engleski

Influence of body mass at birth on the activity of enzymes and biocatalysts concentration in the serum of Large White piglets till weaning

Newborn piglets weight at birth less than 1000 grams are more susceptible to diseases and negative influences of stress factors, slow progress, and therefore cause greater losses than heavier ones. Also, they are physiologically immature and establish postnatal metabolical mechanisms slower than the suckling piglets of normal body mass. The aim of our research was to determine changes in the activity of some enzymes and concentration of biocatalysts in blood serum of suckling piglets. In this study 48 piglets were included: 24 animals with birth weight less than 1000 g (experimental group) and 24 littermates of the same sex with body mass of 1000 g and more (control group). Blood samples were taken on the 1st, 7th, 14th and 21st day of piglets’ life from the vena cava cranialis (1.5 ml) in the BD Vacutainer SST tubes with the gel for biochemical analysis. After centrifugation samples were analyzed using an automatic analyzer Olympus AU 600. In sera of both groups of piglets the activities of creatin kinase and alkaline phosphatase as well as calcium and phosphorus concentrations were higher at weaning while the concentration of magnesium was below the reference value. Also, we found lower activity of creatin kinase in 1- day-old piglets with small birth mass (P<0.05), possibly associated with poor development of the muscle tissue. On the contrary, activity of alanin aminotransferase was significantly higher (P<0.01) in the serum of experimental animals on the 1st day of life. We assume that the observed changes in enzymes activities and biocatalysts concentrations indicate intensive growth and development of newborn animals, especially in the control group of piglets.

Large White ; suckling piglets ; birth weight ; enzymes ; electrolytes

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