engleski

In vitro oxidative stress induced by orthodontic archwires

Background and Purpose: Several metals undergo redox cycling producing free radicals and generating oxidative stress. In vitro oxidative stress of orthodontic archwires made of different alloys was investigated. Materials and Methods: Mouse fibroblast cells L929 were exposed to six types of archwires and the concentration of the oxidative stress marker 8-hydroxy-2'-deoxyguanosine (8-OHdG) in DNA was evaluated. Trypan blue dye was used in the determination of cell viability and cell number. Data Analisis Results: Standard NiTi generated the highest oxidative stress, significantly higher than all wires and controls (p<0.05), and coated NiTi, CuNiTi and CoCr lower than NiTi (p<0.05), but higher than TMA, negative and absolute controls (p<0.05). TMA and SS generated the lowest stress. NiTi induced the lowest viability, lower than negative and absolute control and all wires (p<0.05) except TMA. SS showed the highest viability. NiTi produced the highest inhibition of cell growth, higher than all samples (p<0.05) except positive control and CoCr. The lowest inhibition was observed in SS and TMA, lower than NiTi, CoCr and positive control (p<0.05). Conclusion: SS archiwire presents the highest and NiTi the lowest biocompatibility. CuNiTi, rhodium coated NiTi and TMA show better biocompatibility in comparison to conventional NiTi which makes them a proper alternative in leveling and finishing.

oxidative stress; archwires

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