engleski

Roles of RecQ, UvrD and HelD helicases in the RecF recombination pathway of Escherichia coli

DNA helicases play an important role during initiation of homologous recombination by generating recombinogenic 3’ single-stranded DNA ends. Recombination in recBCD sbcB sbcC(D) mutants of Escherichia coli proceeds via RecF pathway, which is thought to require RecQ, UvrD and HelD helicases at its initial stage. Previous studies suggested that depletion of all three helicases totally abolishes the RecF pathway. The present study (re)examines the roles of these helicases in transductional recombination, and in recombinational repair of UV-induced DNA damage in the RecF pathway.

DNA repair; Escherichia coli; helicases; nucleases; recombination; RecF pathway

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