engleski

Plum Pox Virus on Sour Cherry in Croatia

Plum pox virus (PPV) has been routinely screened in Croatian nurseries since 1988 by ELISA. From 2004 onwards, IC-RT-PCR has been introduced to supplement the detection methods in accordance with the EPPO protocol. So far, the PPV strains M, D, and Rec have been detected in the country with the geographic prevalence of PPV-M, especially in the European plum. Sour cherry cultivation has increasing importance in the last decade. Besides commercially available sour cherry varieties grown in the continental part of the country, the tendency is to promote growing of indigenous cultivar Prunus cerasus L. ‘Maraska’ in Dalmatia including the preservation of Maraska gene pool and domestic planting material production. In one of the nurseries specialized for Maraska sour cherry located near Zadar, a routine PPV screening was performed in 2010. Leaf samples were taken form 30 asymptomatic plants and two were found PPV infected by ELISA and IC-RT-PCR. The amplification product obtained by using PPV-C specific primers SoC1/SoC2 resulted in amplification product of expected size (193 bp). The amplicon was cloned into pTZ57R/T vector and inserts from ten recombinant plasmids were sequenced. The genetic structure of this PPV-SoC isolate was homogeneous with all ten amplicon sequences sharing 100% sequence identity. Phylogenetic analysis of 177 nucleotide-long fragments, obtained after removing the primer sequences from amplicons, showed that PPV-SoC isolate from Maraska clusters with other SoC isolates. Interestingly, it also shares 100% nucleotide identity with the prototype sour cherry isolate (GenBank Acc. No. AY184478) originating from Moldavia. This is the first report of PPV-C strain in Croatia as well as the first report of PPV in sour cherry.

PPV-C; Marasca; RT-PCR; SoC isolate

nije evidentirano